How do I “reinoculate” a petri dish with new bacteria?

How do I “reinoculate” a petri dish with new bacteria?

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Hypothetical question, intended to find a general procedure rather than case-specific recommendations:

I have a petri dish full of growth medium with a large population of gram-negative, anaerobic bacteria of numerous types growing in it. How would I go about replacing the existing population with lactobacillus and streptococcus strains?


  • The growth medium cannot be washed out of the petri dish
  • The petri dish cannot be treated with anything a human body can't handle, i.e. no blasting with bleach
  • After the procedure is complete, the original bacteria should have little to no chance of returning, even given months or years in the petri dish